8IH8

anti-sigmaF factor and Anti-sigmaF factor antagonist complex(usfx-RsfB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	50.425, 77.928, 133.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.650 - 2.000
R-factor	0.1824
Rwork	0.180
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.390
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.057
Number of reflections	36351	21537
<I/σ(I)>	42.7
Completeness [%]	99.3
Redundancy	12.7
CC(1/2)		0.150

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05M HEPES pH7.5 0.2M KCl 35.5% v/v pentaerythritol propoxylate (5/4 PO/OH)