8IH7
AmnG-AmnH complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.029, 252.288, 60.785 |
Unit cell angles | 90.00, 114.94, 90.00 |
Refinement procedure
Resolution | 20.110 - 2.480 |
R-factor | 0.1749 |
Rwork | 0.173 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.937 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.070 | 2.550 |
High resolution limit [Å] | 2.480 | 2.480 |
Number of reflections | 52921 | 52921 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.996 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 0.2M magnesium chloride hexahydrate,0.2 M Tris 8.5,20% PEG 3350 |