8IGV
Hexameric Ring Complex of Engineered V1-ATPase bound to 5 ADPs: A3(De)3_(ADP-Pi)1cat(ADP)2cat,2non-cat
This is a non-PDB format compatible entry.
Replaces: 7CORExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-10 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 119.667, 126.736, 123.649 |
Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
Resolution | 48.380 - 3.150 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.26810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.939 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.380 | 3.230 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.210 | 1.110 |
Rpim | 0.087 | 0.490 |
Number of reflections | 63835 | 4482 |
<I/σ(I)> | 7.7 | 2 |
Completeness [%] | 100.0 | |
Redundancy | 7.1 | |
CC(1/2) | 0.984 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl (pH 8.5), 24% PEG 3350, 0.2 M Ammonium Acetate |