8IGU

Hexameric Ring Complex of Engineered V1-ATPase: A3(De)3_empty

This is a non-PDB format compatible entry.

Replaces:  7COP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	122.449, 122.653, 128.704
Unit cell angles	90.00, 90.74, 90.00

Refinement procedure

Resolution	44.640 - 2.770
R-factor	0.2187
Rwork	0.216
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.640	2.820
High resolution limit [Å]	2.770	2.770
Rmerge	0.098	0.876
Rpim	0.040	0.380
Number of reflections	96837	4764
<I/σ(I)>	14.2	2.1
Completeness [%]	100.0	100
Redundancy	7	7.3
CC(1/2)	0.998	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl (pH 8.5), 24% PEG 3350 and 0.2 M Ammonium Acetate