8IGD
The crystal structure of the minimal interaction domains of DRB7.2:DRB4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-11-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 4 |
| Unit cell lengths | 93.940, 93.940, 102.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.900 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.035 |
| Data reduction software | iMOSFLM (v7.2.2) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX (1.16_3549) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 3.040 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.068 | 0.418 |
| Rmeas | 0.073 | 0.448 |
| Rpim | 0.026 | 0.161 |
| Number of reflections | 9938 | 4256 |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.8 | 7.8 |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, sodium citrate tribasic dihydrate, and isopropanol |
