EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | JEOL CRYO ARM 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | In-column Omega Filter |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.57 |
Software | RELION,PHENIX,SerialEM,CTFFIND,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |