8IE2
Crystal structure of Lactiplantibacillus plantarum GlyRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 155.140, 163.170, 155.280 |
Unit cell angles | 90.00, 119.81, 90.00 |
Refinement procedure
Resolution | 48.630 - 3.600 |
R-factor | 0.2217 |
Rwork | 0.215 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.852 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.690 |
High resolution limit [Å] | 3.600 | 16.100 | 3.600 |
Rmerge | 0.258 | 0.062 | 0.678 |
Rmeas | 0.279 | 0.068 | 0.735 |
Number of reflections | 46387 | 819 | 691 |
<I/σ(I)> | 6.82 | ||
Completeness [%] | 59.5 | ||
Redundancy | 6.8 | ||
CC(1/2) | 0.989 | 0.997 | 0.746 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% PEG3350, 4% Tacsimate pH 5.0 |