8IDZ

Beta-glucosidase BglPcC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-26
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	182.607, 133.842, 69.843
Unit cell angles	90.00, 102.62, 90.00

Refinement procedure

Resolution	37.450 - 4.000
R-factor	0.2529
Rwork	0.250
R-free	0.30180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.574
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.450	4.146
High resolution limit [Å]	4.000	4.000
Rmerge	0.065	0.200
Rmeas	0.091	0.283
Rpim	0.065	0.200
Number of reflections	10169	206
<I/σ(I)>	7.77	4.46
Completeness [%]	73.0
Redundancy	1.86
CC(1/2)	0.993	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,0.2M Li2SO4