8IDW
Crystal structure of Fic protein from Mycoplasma mycoides in complex with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.506, 68.299, 121.006 |
Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
Resolution | 34.765 - 2.304 |
R-factor | 0.2058 |
Rwork | 0.203 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.450 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.074 | 0.400 |
Rmeas | 0.085 | 0.479 |
Number of reflections | 41783 | 6076 |
<I/σ(I)> | 11 | 2.8 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 35.9 | 25.8 |
CC(1/2) | 0.997 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Magnesium chloride, 0.1 M BIS-TRIS, 25% w/v Polyethylene glycol 3,350 |