8IDW
Crystal structure of Fic protein from Mycoplasma mycoides in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.506, 68.299, 121.006 |
| Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
| Resolution | 34.765 - 2.304 |
| R-factor | 0.2058 |
| Rwork | 0.203 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.400 |
| Rmeas | 0.085 | 0.479 |
| Number of reflections | 41783 | 6076 |
| <I/σ(I)> | 11 | 2.8 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 35.9 | 25.8 |
| CC(1/2) | 0.997 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Magnesium chloride, 0.1 M BIS-TRIS, 25% w/v Polyethylene glycol 3,350 |
