8ID7
Crystal structure of YbiW in complex with 1,5-anhydroglucitol-6-phosphate in Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 116.480, 207.170, 208.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.482 - 2.650 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.008 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.270 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.193 | 1.589 |
| Rmeas | 0.211 | 1.722 |
| Rpim | 0.085 | 0.660 |
| Total number of observations | 232578 | 18068 |
| Number of reflections | 36549 | 2668 |
| <I/σ(I)> | 7.7 | 1.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 | 6.8 |
| CC(1/2) | 0.991 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M sodium chloride, 0.1M Tris, pH 8.0, 25% (w/v) PEG3350, 10 mM 1,5-anhydroglucitol-6-phosphate |
