8ICF
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SEVEN BASE PAIRS OF DNA; SOAKED IN THE PRESENCE OF DATP (10 MILLIMOLAR) AND MGCL2 (50 MILLIMOLAR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1995-03-14 |
Detector | SDMS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 178.875, 57.713, 48.385 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.185 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 8icb |
RMSD bond length | 0.017 |
RMSD bond angle | 3.100 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT (V. 5-D) |
Refinement software | TNT (5-D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 10998 | |
Completeness [%] | 94.0 | 93 |
Redundancy | 1.8 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A COCRYSTAL OF HUMAN DNA POLYMERASE BETA COMPLEXED WITH 7 BASE PAIRS OF DNA (SEE ENTRY 9ICJ AND REFERENCE 1) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 24 HOURS: PEG 3350, 16% MES, 100 MILLIMOLAR, PH 6.5 NACL, 150 MILLIMOLAR DATP, 10 MILLIMOLAR MGCL2, 50 MILLIMOLAR SEE REFERENCE 2 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE. |