8ICD

REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	105.100, 105.100, 150.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.500
R-factor	0.168 *
Rwork	0.168
RMSD bond length	0.016
RMSD bond angle	3.100
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.093 *
Total number of observations	62251 *
Number of reflections	26412 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4 *		taken from Hurley, J.H. et al (1989). Proc. Natl. Acad. Sci. USA, 86, 8635-8639. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	28 (mg/ml)
2	1	1	ammonium sulfate	34 (%sat)
3	1	1		100 (mM)
4	1	1		35 (mM)
5	1	1	citric acid	9 (mM)
6	1	1	dithiothreitol	0.2 (mM)