8IC9
Lys48-linked K48C-diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2021-07-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9000 |
Spacegroup name | P 1 |
Unit cell lengths | 86.790, 31.191, 25.272 |
Unit cell angles | 90.00, 106.93, 88.65 |
Refinement procedure
Resolution | 19.641 - 1.250 |
Rwork | 0.186 |
R-free | 0.21880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.574 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.641 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.062 | 0.554 |
Number of reflections | 66682 | 11849 |
<I/σ(I)> | 6.7 | 2.7 |
Completeness [%] | 95.1 | 93.6 |
Redundancy | 3.6 | |
CC(1/2) | 0.992 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 38% 2-methyl-2,4-pentanediol and 50 mM sodium citrate (pH 4.0) |