8IC8

Exo-alpha-D-arabinofuranosidase from Microbacterium arabinogalactanolyticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	131.109, 207.538, 253.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 2.420
R-factor	0.1915
Rwork	0.190
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.240	2.460
High resolution limit [Å]	2.420	2.420
Rmerge	0.147	0.616
Rmeas	0.158	0.661
Rpim	0.057	0.239
Number of reflections	264416	12946
<I/σ(I)>	9.9	2.8
Completeness [%]	100.0	100
Redundancy	7.6	7.6
CC(1/2)	0.995	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaCl, 0.1 M phosphate citrate, 20% PEG8000