8IC7
exo-beta-D-arabinofuranosidase ExoMA2 from Microbacterium arabinogalactanolyticum in complex with beta-D-arabinofuranose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-28 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.763, 97.249, 139.681 |
Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
Resolution | 45.870 - 1.350 |
R-factor | 0.1601 |
Rwork | 0.159 |
R-free | 0.18244 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.651 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless (0.7.9) |
Phasing software | MOLREP (11.9.02) |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.620 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.056 | 0.965 |
Rpim | 0.036 | 0.629 |
Number of reflections | 382283 | 18879 |
<I/σ(I)> | 9.5 | 1 |
Completeness [%] | 99.9 | |
Redundancy | 3.4 | |
CC(1/2) | 0.988 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293.15 | 0.2 M MgCl2, 0.1 M CHES-NaOH (pH 8.9), 14% PEG8000, 100 mM D-arabinose |