8I8Z

Structure of flavone 4'-O-glucoside 7-O-glucosyltransferase from Nemophila menziesii, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-03-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	55.799, 75.151, 117.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.380 - 2.400
R-factor	0.2035
Rwork	0.198
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.971
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.380	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.153
Number of reflections	20008	1920
<I/σ(I)>	13.9
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)		0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG 4000, Tris-HCl, Lithium Sulfate