8I86

Crystal structure of Cph001-D189N in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-07
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	82.155, 86.784, 87.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 2.230
R-factor	0.23082
Rwork	0.228
R-free	0.28845
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.910
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.370
High resolution limit [Å]	2.230	2.230
Rmerge	0.063	0.437
Number of reflections	29326	4318
<I/σ(I)>	11.03	2.36
Completeness [%]	99.5	98.6
Redundancy	3.81
CC(1/2)	0.997	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	Sodium acetate trihydrate, Polyethylene glycol 4,000, Glycerol