8I81

Crystal structure of horse spleen L-ferritin A115G mutant at -180deg Celsius.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2022-08-02
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	181.653, 181.653, 181.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.705 - 1.500
Rwork	0.152
R-free	0.16840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.706
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.705	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.058	0.470
Rmeas	0.061	0.511
Number of reflections	41546	2034
<I/σ(I)>	26.2	3.6
Completeness [%]	100.0
Redundancy	9.6
CC(1/2)	0.999	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM)