8I7E

Crystal structure of Glyceraldehyde 3-phosphate dehydrogenase from Salmonella typhi at 2.05A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-24
Detector	RIGAKU
Wavelength(s)	1.54179
Spacegroup name	C 2 2 21
Unit cell lengths	90.901, 107.952, 122.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.980 - 2.050
R-factor	0.2467
Rwork	0.244
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.980	2.123
High resolution limit [Å]	2.050	2.050
Number of reflections	37312	37312
<I/σ(I)>	1.68
Completeness [%]	97.8
Redundancy	14.6
CC(1/2)	0.979	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.2 M Imidazole pH 7.0, 40% PEG