8I77

Crystal structure of horse spleen L-ferritin A115V mutant at -180deg Celsius.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2022-08-02
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.541838
Spacegroup name	F 4 3 2
Unit cell lengths	181.749, 181.749, 181.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.721 - 1.500
Rwork	0.160
R-free	0.17690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.012
RMSD bond angle	1.678
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.721	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.075	0.742
Rmeas	0.088	0.836
Number of reflections	41611	2029
<I/σ(I)>	13.8
Completeness [%]	100.0
Redundancy	7.1
CC(1/2)	0.999	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM)