8I6K

Structure of hMNDA HIN with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-24
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	40.652, 40.652, 138.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.070 - 2.400
R-factor	0.202
Rwork	0.199
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.330
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.070	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.038	0.356
Rmeas	0.042	0.422
Rpim	0.019	0.223
Number of reflections	9936	955
<I/σ(I)>	30.5	2.94
Completeness [%]	99.3	100
Redundancy	4.8	3.4
CC(1/2)	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.1 M Sodium malonate pH 8.0, 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 1000