8I6H

Crystal structure of the African swine fever virus DNA sliding clamp (selenomethionine form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	H 3
Unit cell lengths	146.637, 146.637, 92.934
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.854 - 2.292
R-factor	0.1837
Rwork	0.181
R-free	0.22880
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.078
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.370
High resolution limit [Å]	2.290	4.930	2.290
Rmerge	0.113	0.081	0.707
Rmeas	0.121	0.087	0.760
Rpim	0.043	0.031	0.273
Total number of observations	267233
Number of reflections	33401	3344	3359
<I/σ(I)>	9.9
Completeness [%]	99.9	100	99.5
Redundancy	8	7.7	6.9
CC(1/2)		0.995	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	Tris-HCl, PEG 3350, Ammonium Acetate