8I6G

Crystal structure of the African swine fever virus DNA sliding clamp (native form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	H 3
Unit cell lengths	146.767, 146.767, 94.491
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.386 - 1.790
R-factor	0.2045
Rwork	0.203
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8i6h
RMSD bond length	0.007
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.850
High resolution limit [Å]	1.790	3.860	1.790
Rmerge	0.044	0.034	0.536
Rmeas	0.049	0.038	0.630
Rpim	0.022	0.017	0.323
Total number of observations	332433
Number of reflections	69816	7151	5902
<I/σ(I)>	17.5
Completeness [%]	97.1	99.3	81.7
Redundancy	4.8	5	3.3
CC(1/2)		0.998	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	Tris-HCl, PEG 3350, Ammonium Acetate