8I6G
Crystal structure of the African swine fever virus DNA sliding clamp (native form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 146.767, 146.767, 94.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.386 - 1.790 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8i6h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 3.860 | 1.790 |
| Rmerge | 0.044 | 0.034 | 0.536 |
| Rmeas | 0.049 | 0.038 | 0.630 |
| Rpim | 0.022 | 0.017 | 0.323 |
| Total number of observations | 332433 | ||
| Number of reflections | 69816 | 7151 | 5902 |
| <I/σ(I)> | 17.5 | ||
| Completeness [%] | 97.1 | 99.3 | 81.7 |
| Redundancy | 4.8 | 5 | 3.3 |
| CC(1/2) | 0.998 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 289 | Tris-HCl, PEG 3350, Ammonium Acetate |
