8I4D
X-ray structure of a L-rhamnose-alpha-1,4-D-glucuronate lyase from Fusarium oxysporum 12S, L-Rha complex at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.313, 65.230, 108.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.650 - 1.060 |
| R-factor | 0.1723 |
| Rwork | 0.172 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yqs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.630 | 1.080 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.103 | 1.075 |
| Rmeas | 0.107 | 1.124 |
| Rpim | 0.029 | 0.323 |
| Number of reflections | 180680 | 8886 |
| <I/σ(I)> | 11.9 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 11.9 |
| CC(1/2) | 0.998 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PROTEIN SOLUTION : PROTEIN 24MG/ML, 20MM TRIS-DCL (PD 8.0), RESERVOIR SOLUTION : 36 % (W/V) PEG 1500, 0.1 M TRIS-DCL (PD 8.5), 0.1 M L-RHA |
