8I3Y
Crystal structure of ASCT from Trypanosoma brucei in complex with Succinyl-CoA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.801, 163.654, 186.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.400 |
| R-factor | 0.19439 |
| Rwork | 0.192 |
| R-free | 0.24815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.128 | 0.721 |
| Rpim | 0.138 | 0.779 |
| Number of reflections | 72921 | 11459 |
| <I/σ(I)> | 12.8 | 3.2 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 6.5 | 6.2 |
| CC(1/2) | 0.997 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.05M HEPES-NAOH BUFFER, 18% (W/V) PEG 3350, 0.35M CaCl2 |
