8I3N
crystal structure of yeast cytosine deaminase mutant yCD-RQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97914 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.669, 64.470, 83.092 |
Unit cell angles | 90.00, 97.97, 90.00 |
Refinement procedure
Resolution | 82.290 - 1.730 |
R-factor | 0.2032 |
Rwork | 0.203 |
R-free | 0.21780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.609 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.290 | 1.820 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.070 | 0.480 |
Rmeas | 0.083 | 0.597 |
Rpim | 0.043 | 0.350 |
Total number of observations | 25357 | |
Number of reflections | 65362 | 9660 |
<I/σ(I)> | 11.5 | 2.5 |
Completeness [%] | 96.9 | |
Redundancy | 3.5 | 2.6 |
CC(1/2) | 0.996 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.15M ammounium acetate, 27%-29% PEG3350 |