8I3J

Crystal structure of human inner-arm dynein heavy chain d stalk and microtubule binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97938
Spacegroup name	P 61 2 2
Unit cell lengths	147.468, 147.468, 85.075
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.570 - 2.690
R-factor	0.2582
Rwork	0.258
R-free	0.26790
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.422
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.690	2.690
Rmerge	0.123
Rmeas	0.125	2.062
Rpim	0.020
Number of reflections	28811	4588
<I/σ(I)>	24.83	2.12
Completeness [%]	98.7	98.3
Redundancy	20.9	19.4
CC(1/2)	1.000	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Potassium sodium tartrate tetrahydrate, PEG 3350