8I3F

Crystal structure of Rco1-Eaf3 with peptide of histone H3 N-terminal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	82.513, 75.546, 73.421
Unit cell angles	90.00, 114.19, 90.00

Refinement procedure

Resolution	28.237 - 1.620
R-factor	0.1684
Rwork	0.167
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.187
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.490	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.037	0.485
Number of reflections	53957	2480
<I/σ(I)>	24.6	2.3
Completeness [%]	99.6
Redundancy	6.2	3.9
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M MgCl2, 0.1 M tris pH 8.5, 25% w/v PEG 4000, 0.2 M NDSB-201