8I2K
Crystal structure of transcriptional regulator pvrA from Pseudomonas aeruginosa.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-10 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.5 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.298, 96.584, 104.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.368 - 2.303 |
R-factor | 0.1975 |
Rwork | 0.192 |
R-free | 0.25400 |
Structure solution method | MIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.233 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | AutoSol |
Refinement software | PHENIX (v1.14) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.960 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 21122 | 2059 |
<I/σ(I)> | 27.8 | |
Completeness [%] | 99.2 | |
Redundancy | 18.7 | |
CC(1/2) | 0.999 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 3000, 100mM Tris, 200mM Nacl |