8I2E
Crystal structure of Bacillus subtilis LytE in complex with IseA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 87.010, 87.010, 217.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.890 - 3.200 |
| R-factor | 0.2359 |
| Rwork | 0.234 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.343 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.195 | 0.852 |
| Rpim | 0.091 | 0.375 |
| Number of reflections | 14448 | 2547 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 293 | 25% (w/v) PEG1500, 100mM SPG buffer pH 5.5 |
