8I23
Clostridium thermocellum RNA polymerase transcription open complex with SigI1 and its promoter
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.03 |
| Software | PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
