8I16

Crystal structure of the selenomethionine (SeMet)-derived Cas12g (D513A) mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	64.070, 95.120, 167.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.860 - 2.240
R-factor	0.2262
Rwork	0.224
R-free	0.26849
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.665
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.13-2998)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.860	2.300
High resolution limit [Å]	2.240	2.240
Rmerge	0.091
Number of reflections	49819	3596
<I/σ(I)>	17	2.3
Completeness [%]	99.4	98.2
Redundancy	13
CC(1/2)	0.997	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M BIS-TRIS (pH 6.5) and 25% polyethylene glycol 3,350 (w/v)