8I11
Crystal structure of LOV1 domain of phototropin from Klebsormidium nitens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.88560 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 53.868, 53.868, 137.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.340 - 1.855 |
Rwork | 0.193 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.661 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.340 | 5.000 |
High resolution limit [Å] | 1.850 | 1.855 |
Number of reflections | 17804 | 858 |
<I/σ(I)> | 17.7 | |
Completeness [%] | 99.7 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289.15 | magnesium Sulfate hydrate, HEPES sodium pH 7.0, lithium sulfate monohydrate |