8I08
Crystal structure of Escherichia coli glyoxylate carboligase quadruple mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 188.565, 188.565, 246.486 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.920 - 1.910 |
R-factor | 0.2268 |
Rwork | 0.225 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.996 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (v2.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.940 |
High resolution limit [Å] | 1.910 | 5.180 | 1.910 |
Rmerge | 0.989 | 0.074 | 0.964 |
Total number of observations | 4914883 | ||
Number of reflections | 337289 | 32837 | 32608 |
<I/σ(I)> | 2.1 | ||
Completeness [%] | 99.6 | 100 | 99.3 |
Redundancy | 7.5 | 12.2 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 mM Tris-HCl (pH 8.0), 0.5 M sodium chloride, 40 mM dithiothreitol, 0.5% (v/v) polyethylene glycol 6000 (PEG6K) |