8I05
Crystal structure of Escherichia coli glyoxylate carboligase double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 189.172, 189.172, 246.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.810 - 2.090 |
R-factor | 0.2104 |
Rwork | 0.209 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.992 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (v2.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.090 | 5.700 | 2.100 |
Rmerge | 0.999 | 0.067 | 1.697 |
Number of reflections | 253025 | 25087 | 22945 |
<I/σ(I)> | 2.7 | ||
Completeness [%] | 96.3 | 99.4 | 91.1 |
Redundancy | 10.1 | 13.7 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 mM Tris-HCl (pH 8.0), 0.5 M sodium chloride, 40 mM dithiothreitol, 0.5% (v/v) polyethylene glycol 6000 (PEG6K) |