8HZZ
GuApiGT (UGT79B74)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.159, 128.676, 47.564 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 19.660 - 2.200 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.959 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.660 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 48728 | 2332 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 5.25, 28% w/v polyethylene glycol 3,350. |
