8HZM
A new fluorescent RNA aptamer bound with N, manganese soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.240 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.694, 46.871, 56.722 |
| Unit cell angles | 90.00, 113.70, 90.00 |
Refinement procedure
| Resolution | 39.140 - 2.500 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8hze |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.116 | 0.115 | 0.561 |
| Rmeas | 0.126 | 0.125 | 0.655 |
| Rpim | 0.049 | 0.047 | 0.329 |
| Total number of observations | 37919 | ||
| Number of reflections | 6395 | 347 | 260 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 97.1 | 99.4 | 78.1 |
| Redundancy | 5.9 | 6.4 | 3.2 |
| CC(1/2) | 0.984 | 0.988 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.05 M Magnesium Chloride, 0.1 M HEPES pH7.5, 30%polyethylene glycol monomethyl ether 550, 0.6% 1,6-diaminohexane |
