8HZK
A new fluorescent RNA aptamer bound with N, iridium hexammine soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.102 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.893, 44.022, 58.304 |
Unit cell angles | 90.00, 112.94, 90.00 |
Refinement procedure
Resolution | 37.110 - 2.000 |
R-factor | 0.2046 |
Rwork | 0.203 |
R-free | 0.23990 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.178 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.125 | 0.106 | 0.245 |
Rmeas | 0.149 | 0.128 | 0.291 |
Rpim | 0.081 | 0.070 | 0.156 |
Total number of observations | 77364 | ||
Number of reflections | 11887 | 2259 | 2312 |
<I/σ(I)> | 8 | ||
Completeness [%] | 98.9 | 98.1 | 99.3 |
Redundancy | 3.4 | 3.3 | 3.4 |
CC(1/2) | 0.962 | 0.972 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.05 M magnesium chloride, 0.1 M HEPES pH 7.5, 30% polyethylene glycol monomethyl ether 550, 0.6% 1,6-diaminohexane |