8HZF

A new fluorescent RNA aptamer bound with N565

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1020
Spacegroup name	C 1 2 1
Unit cell lengths	84.634, 47.237, 56.170
Unit cell angles	90.00, 115.26, 90.00

Refinement procedure

Resolution	40.200 - 2.700
R-factor	0.186
Rwork	0.182
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hze
RMSD bond length	0.006
RMSD bond angle	1.370
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.156	0.072	1.268
Rmeas	0.169	0.079	1.376
Rpim	0.065	0.031	0.528
Number of reflections	5579	580	557
<I/σ(I)>	2.8
Completeness [%]	98.9	98.1	99.6
Redundancy	6.7	6.4	6.7
CC(1/2)		0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Bicine pH8.5, 65%MPD