8HZE

A new fluorescent RNA aptamer bound with N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97892
Spacegroup name	C 1 2 1
Unit cell lengths	76.151, 45.870, 57.116
Unit cell angles	90.00, 115.08, 90.00

Refinement procedure

Resolution	38.190 - 1.590
R-factor	0.2011
Rwork	0.200
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.965
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.590	3.450	1.600
Rmerge	0.098	0.078	0.581
Rmeas	0.107	0.085	0.628
Rpim	0.041	0.033	0.237
Number of reflections	23824	2424	2370
<I/σ(I)>	6.2
Completeness [%]	99.6	98.6	100
Redundancy	6.7	6.7	6.9
CC(1/2)	1.000	0.995	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05 M magnesium chloride, 0.1 M HEPES pH 7.5, 30% polyethylene glycol monomethyl ether 550, 0.6% 1,6-diaminohexane