8HZD

A new fluorescent RNA aptamer bound with N618

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.102
Spacegroup name	C 1 2 1
Unit cell lengths	77.494, 46.461, 56.251
Unit cell angles	90.00, 113.81, 90.00

Refinement procedure

Resolution	28.160 - 1.870
R-factor	0.213
Rwork	0.211
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hze
RMSD bond length	0.005
RMSD bond angle	1.030
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.860	1.970
High resolution limit [Å]	1.870	1.870
Rmerge	0.060	0.648
Rmeas	0.065	0.713
Rpim	0.026	0.279
Number of reflections	14913	3566
<I/σ(I)>	14.4
Completeness [%]	96.9
Redundancy	6.4
CC(1/2)	0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05 M Magnesium Chloride, 0.1 M HEPES pH7.5, 30%(w/v) PEGmm550, 0.001 M GSH