8HZ8
Structure of PPIA in complex with the peptide of NRF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2020-12-25 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.490, 52.326, 88.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.700 - 1.810 |
Rwork | 0.234 |
R-free | 0.24780 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.446 | 1.950 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.360 | |
Number of reflections | 16105 | 15285 |
<I/σ(I)> | 67.13 | |
Completeness [%] | 99.0 | |
Redundancy | 19.5 | |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, Hepes, TCEP |