8HYN

Bacterial STING from Riemerella anatipestifer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	53.265, 60.121, 118.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.957 - 2.089
Rwork	0.189
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.395
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.089	2.089
Rmerge	0.042	0.301
Number of reflections	22890	2235
<I/σ(I)>	43.126
Completeness [%]	98.3
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Imidazole/MES pH 6.5, 0.1 M carboxylic acids, 20% v/v Ethylene glycol, 10 % w/v PEG 8000