8HYL
Crystal structure of DO1 Fv-clasp fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.899995 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.277, 86.207, 112.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.000 |
R-factor | 0.2442 |
Rwork | 0.241 |
R-free | 0.29810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.316 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.182 | 2.000 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 92507 | 14760 |
<I/σ(I)> | 5.89 | |
Completeness [%] | 99.1 | |
Redundancy | 3.38 | |
CC(1/2) | 0.993 | 0.460 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M Tris-HCl (pH 7.5), 25%[w/v] PEG 3350, 0.2 M lithium nitrate |