8HY9
Bacterial STING from Riemerella anatipestifer in complex with 3'3'-c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.510, 40.990, 36.196 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 22.805 - 1.462 |
| Rwork | 0.181 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ebd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.437 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.510 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.054 | 0.489 |
| Number of reflections | 24248 | 2302 |
| <I/σ(I)> | 30.227 | 2.92 |
| Completeness [%] | 99.3 | 95 |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Tris pH 8.5, 0.2 M calcium chloride dihydrate, 40 % v/v PEG 4000 |
