8HXS
Small_spotted catshark CD8alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 291.15 |
Detector technology | CCD |
Collection date | 2020-08-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.384, 40.446, 32.828 |
Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
Resolution | 35.324 - 1.350 |
Rwork | 0.212 |
R-free | 0.21610 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 20802 | |
<I/σ(I)> | 2.19 | |
Completeness [%] | 99.2 | |
Redundancy | 12.4 | |
CC(1/2) | 0.960 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.5 | 30% (w/v) PEG3000 and 100mM CHES/Sodium hydroxide pH9.5 |