8HXR

Nanobody2 in complex with human BCMA ECD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 41
Unit cell lengths	80.062, 80.062, 78.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.030 - 2.700
Rwork	0.240
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.656
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.700	2.700
Number of reflections	13801	2206
<I/σ(I)>	6.25
Completeness [%]	99.8
Redundancy	4.6
CC(1/2)	0.973	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.8 M Sodium acetate trihydrate, 0.1 M Bis-Tris Propane pH7.0