8HXQ

Nanobody1 in complex with human BCMA ECD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	66.773, 66.773, 174.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.220 - 2.400
Rwork	0.227
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.670	2.450
High resolution limit [Å]	2.390	7.550	2.390
Rmerge	0.136	0.088	1.371
Rmeas	0.142	0.093
Number of reflections	16468	383	1142
<I/σ(I)>	11.33
Completeness [%]	99.6
Redundancy	12.6
CC(1/2)	0.994	0.991	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	293	0.2 M Ammonium sulfate, 20% w/v Polyethylene glycol 3350, pH6.1