8HXQ
Nanobody1 in complex with human BCMA ECD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.773, 66.773, 174.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.220 - 2.400 |
Rwork | 0.227 |
R-free | 0.27350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.084 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 10.670 | 2.450 |
High resolution limit [Å] | 2.390 | 7.550 | 2.390 |
Rmerge | 0.136 | 0.088 | 1.371 |
Rmeas | 0.142 | 0.093 | |
Number of reflections | 16468 | 383 | 1142 |
<I/σ(I)> | 11.33 | ||
Completeness [%] | 99.6 | ||
Redundancy | 12.6 | ||
CC(1/2) | 0.994 | 0.991 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 0.2 M Ammonium sulfate, 20% w/v Polyethylene glycol 3350, pH6.1 |