8HX3
crystal structure of secreted coleopteran active protein (Sip)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.020, 233.987, 111.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.540 - 2.280 |
R-factor | 0.2415 |
Rwork | 0.240 |
R-free | 0.27800 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.563 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.540 | 2.366 |
High resolution limit [Å] | 2.280 | 2.284 |
Number of reflections | 30454 | 2998 |
<I/σ(I)> | 4.6 | |
Completeness [%] | 97.9 | |
Redundancy | 1.61 | |
CC(1/2) | 0.988 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG3350, 0.2M sodium chloride, 0.1M Hepes PH 7.0 |