8HX3

crystal structure of secreted coleopteran active protein (Sip)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	51.020, 233.987, 111.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.540 - 2.280
R-factor	0.2415
Rwork	0.240
R-free	0.27800
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CRANK2
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.540	2.366
High resolution limit [Å]	2.280	2.284
Number of reflections	30454	2998
<I/σ(I)>	4.6
Completeness [%]	97.9
Redundancy	1.61
CC(1/2)	0.988	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG3350, 0.2M sodium chloride, 0.1M Hepes PH 7.0