8HW3
Limosilactobacillus reuteri N1 GtfB-acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-12 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 204.770, 100.620, 106.670 |
| Unit cell angles | 90.00, 108.17, 90.00 |
Refinement procedure
| Resolution | 47.110 - 2.660 |
| R-factor | 0.21456 |
| Rwork | 0.212 |
| R-free | 0.26217 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.307 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 2.730 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.099 | 0.501 |
| Number of reflections | 58618 | 4439 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 4% v/v MPD, 0.1 M Citric acid pH 3.5, 20% PEG 1500 |
